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c/coa·submitted 1 year ago by u/aleksi_eriksen

purity — 9 things I got wrong before I got it right

Explainerbranch of 11 comments

Thinking out loud about this: purity — 9 things I got wrong before I got it right. How to ask for a batch record without it turning into a negotiation. One email, three requests: the batch-specific certificate for lot X, the method line behind it, and the storage condition the stability statement was written against.…

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11 comments, started 1 year ago
u/coa_janitorMOD288 points·1 year ago

Your name and address edited out of the screenshot above. The batch details are untouched and should stay.

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u/blunt_coldbox2969 points·1 year ago

ask for the storage condition the stability statement was written against

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u/freya_baptista40 points·1 year ago

Did you get the chromatogram or only the summary line?

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u/soren_nkemelu19 points·1 year ago·edited

Did you get the chromatogram or only the summary line?

freya_baptista has the distinction right — one is a fraction of the detector signal, the other is a fraction of the mass.

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u/ignacio_silva48 points·1 year ago

My first certificate had a compound name, a percentage and a logo. I thought that was normal. It is not, and now I know what to ask for.

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u/purity_pedantanalytical40 points·1 year ago·edited

ask for the storage condition the stability statement was written against

This is the sentence to take away. The rest of the thread is footnotes to it.

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u/sunday_shot_sal21 points·1 year ago

the related substances line is where the story is

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u/aleksi_eriksenOP15 points·1 year ago

Correction: that is net peptide content, not purity. Different number, different meaning, and the document says so if you read the row label.

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u/aleksi_eriksenOP120 points·1 year ago

Area percent or net peptide content? They answer different questions.

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u/hassan_chowdhury175 points·1 year ago

Your name and address edited out of the screenshot above.

Agreed, and the practical version is: ask for the itemised table. If it exists, you get it.

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u/andres_restrepo339 points·1 year ago

Ask what the stability statement was written against — temperature, light, and whether it was the lyophilised powder or the reconstituted solution. Those are three different claims.

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About c/coa

How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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