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c/coa·submitted 7 months ago by u/sofia_ferreira

three years of related substances threads, summarised so you do not have to read them

Explainerbranch of 8 comments

three years of related substances threads, summarised so you do not have to read them, and I am aware this is a minority view on this board. UV at 214nm sees the peptide bond and picks up almost everything; at 280nm you are looking at aromatics only. Which wavelength the number came from is part of the number. LC-MS…

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8 comments, started 7 months ago
u/liv_nkemelu108 points·7 months ago

Right, and net peptide content is where the counterion and the water go. Both numbers can be honest and very different.

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u/sofia_ferreiraOP69 points·7 months ago

the related substances line is where the story is

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u/noor_cardoso159 points·7 months ago

My first certificate had a compound name, a percentage and a logo. I thought that was normal. It is not, and now I know what to ask for.

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u/andres_restrepo0 points·7 months ago

My first certificate had a compound name, a percentage and a logo.

noor_cardoso has the distinction right — one is a fraction of the detector signal, the other is a fraction of the mass.

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u/certified_referenceMOD65 points·7 months ago

Reminder: a question about a document is not an accusation about a supplier, and posts that blur the two get retitled.

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u/ismael_eriksen18 points·7 months ago·edited

water content is on there and everyone skips it

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[deleted]43 points·7 months ago

[deleted]

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u/sofia_ferreiraOP29 points·7 months ago

the lot on the vial has to match the lot on the page

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About c/coa

How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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